Diluting Primers

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Author
Affiliation

Center for Environmental Studies

Published

September 14, 2013

To dilute primers for PCR, you need to first make a 100µM stock solution. To accomplish this, you find the number of nMol on the tube and add 10X that much water in µl. This is your stock solution. Then for your working solution, dilute it to 10µM and work with that one.