Diluting Primers

A short description of the post.

Rodney Dyer https://dyerlab.org (Center for Environmental Studies)https://ces.vcu.edu

To dilute primers for PCR, you need to first make a 100µM stock solution. To accomplish this, you find the number of nMol on the tube and add 10X that much water in µl. This is your stock solution. Then for your working solution, dilute it to 10µM and work with that one.


For attribution, please cite this work as

Dyer (2013, Sept. 14). The Dyer Laboratory: Diluting Primers. Retrieved from https://dyerlab.github.io/DLabWebsite/posts/2013-09-14-diluting-primers/

BibTeX citation

  author = {Dyer, Rodney},
  title = {The Dyer Laboratory: Diluting Primers},
  url = {https://dyerlab.github.io/DLabWebsite/posts/2013-09-14-diluting-primers/},
  year = {2013}